miRNA-34基因家族进化分析及其在鹅生殖周期内的表达变化

doi:10.11843/j.issn.0366-6964.2016.02.007

Abstract

Abstract:

This experiment was conducted to investigate the miRNA-34 gene family evolutionary process and its expression regularity in goose reproductive cycle.The miRNA-34 genes were searched in literatures and miRBase.It’s genes were located by Ensemble database，and its phylogenetic tree was constructed by MEGA 6.0.In addition，the expression level of miRNA-34s was detected in ovarian tissue of goose reproductive cycle using RT-qPCR.A total 70 sequences in 27 species which indicated the extensive existence of miRNA-34s(miRNA-34a，miRNA-34b and miRNA-34c) in different species.The analysis of gene localization showed all miRNA-34 genes were located on the intergenic region(IGR).miRNA-34b and miRNA-34c shared on the same chromosome and they were closely linked in evolutionary process，which maybe caused by tandem repeats.Besides，the similar expression of miRNA-34s were detected in goose ovary tissues of pre-laying stage，ovulation，oviposition，and the broody phase.More miRNA-34s expression was detected at ovulation，oviposition than that in pre-laying and broody phase period.The miRNA-34s are highly conservative in evolution process，and play a role in regulation reproductive activity in goose.

CLC Number:

S835.2

LIU Ran，ZHANG Hui-zi，YUN Hao-qi，CHEN Yang，HUANG Zheng-yang，ZHANG Yang，XU Qi，CHEN Guo-hong. Molecular Evolution Analyzing of miRNA-34 Gene Family and Its Expression in Goose Reprodutive Cycle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 260-267.

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Molecular Evolution Analyzing of miRNA-34 Gene Family and Its Expression in Goose Reprodutive Cycle

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